PAR1 antibody (AA 251-350)

Catalog No. ABIN728743

This Rabbit Polyclonal antibody specifically detects PAR1 in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Rat and Mouse and has been mentioned in 5+ publications.

Quick Overview for PAR1 antibody (AA 251-350) (ABIN728743)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PAR1 Antibody

Binding Specificity: AA 251-350

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PAR1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PAR1 antibody (ABIN728743)

Posthuma, Posma, Schep, Bender, van Oerle, van der Wal, Ten Cate, Spronk: "Protease-activated receptors are potential regulators in the development of arterial endofibrosis in high-performance athletes." in: Journal of vascular surgery, Vol. 69, Issue 4, pp. 1243-1250, (2019) (PubMed).

Ben Shimon, Zeimer, Shavit Stein, Artan-Furman, Harnof, Chapman, Eisenkraft, Pick, Maggio: "Recovery from trauma induced amnesia correlates with normalization of thrombin activity in the mouse hippocampus." in: PLoS ONE, Vol. 12, Issue 11, pp. e0188524, (2017) (PubMed).

Fan, Chen, Ni, Wang, Sun, Chen, Zheng: "Expression of MMP-1/PAR-1 and patterns of invasion in oral squamous cell carcinoma as potential prognostic markers." in: OncoTargets and therapy, Vol. 8, pp. 1619-26, (2015) (PubMed).

Acton, Miles: "Stimulation of Glia Reveals Modulation of Mammalian Spinal Motor Networks by Adenosine." in: PLoS ONE, Vol. 10, Issue 8, pp. e0134488, (2015) (PubMed).

Liu, Xie, Luo, Chen, Zhou, Xia, Chen, Zhou, Cao, Cao, Zhou: "Identification of FLOT2 as a novel target for microRNA-34a in melanoma." in: Journal of cancer research and clinical oncology, (2014) (PubMed).

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: Thrombin receptor

Background:

Synonyms: TR, HTR, CF2R, PAR1, PAR-1, Proteinase-activated receptor 1, Coagulation factor II receptor, Thrombin receptor, F2R

Background: High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

Gene ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus