Nanog antibody (AA 71-170)

Catalog No. ABIN728758

This Rabbit Polyclonal antibody specifically detects Nanog in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human and Mouse and has been mentioned in 3+ publications.

Quick Overview for Nanog antibody (AA 71-170) (ABIN728758)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Nanog Antibody

Binding Specificity: AA 71-170

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Nanog

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Nanog antibody (ABIN728758)

El-Badawy, Ghoneim, Gabr, Salah, Mohamed, Amer, El-Badri: "Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 254, (2017) (PubMed).

Liu, Zhang, Wang, Yang, Tan: "JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells." in: Acta pharmacologica Sinica, Vol. 36, Issue 9, pp. 1099-106, (2016) (PubMed).

Liu, Li, Liu, Sui, Liu, Xiao, Zheng, Chen, Yao, Xing, Zhou, Li: "A signature for induced pluripotent stem cell-associated genes in colorectal cancer." in: Medical oncology (Northwood, London, England), Vol. 30, Issue 1, pp. 426, (2013) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog

Background:

Synonyms: ENK, ecat4, 2412E2Rik, Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog, Nanog

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Gene ID: 71950

UniProt: Q80Z64

Pathways: Stem Cell Maintenance