ALT antibody (Biotin)

Catalog No. ABIN7630273

This Mouse Monoclonal antibody specifically detects ALT in WB, IHC and ICC. It exhibits reactivity toward Rat.

Quick Overview for ALT antibody (Biotin) (ABIN7630273)

Target: ALT (Alanine Aminotransferase (ALT))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALT antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-ALT Antibody (Biotin)

Purpose: Biotin-Linked Monoclonal Antibody to Alanine Aminotransferase (ALT)

Specificity: The antibody is a mouse monoclonal antibody raised against ALT. It has been selected for its ability to recognize ALT in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: MAA207Ra21Monoclonal Antibody to Alanine Aminotransferase (ALT)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for ALT

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: Alanine Aminotransferase

Background: GPT, ALAT, SGPT, AAT1, GPT1, Serum Glutamic Pyruvic Transaminase, Alanine Transaminase, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1

UniProt: P25409