ENO1 antibody (Biotin)

Catalog No. ABIN7631263

This Rabbit Polyclonal antibody specifically detects ENO1 in WB, IHC and ICC. It exhibits reactivity toward Mouse.

Quick Overview for ENO1 antibody (Biotin) (ABIN7631263)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-ENO1 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Enolase 1 (ENO1)

Specificity: The antibody is a rabbit polyclonal antibody raised against ENO1. It has been selected for its ability to recognize ENO1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: Enolase 1

Background: NNE, ENO1L1, MPB1, PPH, Alpha Enolase, Enolase 1, Phosphopyruvate hydratase, Plasminogen-binding protein, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding

UniProt: P17182