S100A12 antibody (Biotin)

Catalog No. ABIN7633117

This Rabbit Polyclonal antibody specifically detects S100A12 in WB, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for S100A12 antibody (Biotin) (ABIN7633117)

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A12 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-S100A12 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A12 (S100A12)

Specificity: The antibody is a rabbit polyclonal antibody raised against S100A12. It has been selected for its ability to recognize S100A12 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAB080Hu01Polyclonal Antibody to S100 Calcium Binding Protein A12 (S100A12)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for S100A12

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Alternative Name: S100 Calcium Binding Protein A12

Background: CGRP, P6, CAAF1, CAGC, ENRAGE, MRP6, Calgranulin C, Calcium-binding protein in amniotic fluid 1, Extracellular newly identified RAGE-binding, Migration inhibitory factor-related 6

UniProt: P80511

Pathways: Toll-Like Receptors Cascades, S100 Proteins