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beta Actin antibody

The Rabbit Polyclonal anti-beta Actin antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect beta Actin in samples from Human.
Catalog No. ABIN7633970

Quick Overview for beta Actin antibody (ABIN7633970)

Target

See all beta Actin (ACTB) Antibodies
beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This beta Actin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
  • Purpose

    Polyclonal Antibody to Beta Actin (ACTB)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Cow, Dog, Goat, Mouse, Pig, Rat, Sheep

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    RPB340Hu01Recombinant Beta Actin (ACTB)

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.01-5 μg/mL,Immunohistochemistry: 5-50 μg/mL,Immunocytochemistry: 5-50 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.3 mg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    beta Actin (ACTB) (Actin, beta (ACTB))

    Alternative Name

    ACTB

    Background

    Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

    UniProt

    P60709

    Pathways

    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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