beta Actin antibody

Catalog No. ABIN7633972

This Mouse Monoclonal antibody specifically detects beta Actin in WB, IHC, ICC and IP. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Horse and Guinea Pig.

Quick Overview for beta Actin antibody (ABIN7633972)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Horse, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C7

Product Details anti-beta Actin Antibody

Purpose: Monoclonal Antibody to Beta Actin (ACTB)

Specificity: The antibody is a mouse monoclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.

Cross-Reactivity: Goat, Rabbit

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPB340Mi01Recombinant Beta Actin (ACTB)

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background: Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction