APC antibody

Catalog No. ABIN7634108

The Rabbit Polyclonal anti-APC antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect APC in samples from Mouse.

Quick Overview for APC antibody (ABIN7634108)

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-APC Antibody

Purpose: Polyclonal Antibody to Adenomatosis Polyposis Coli Protein (APC)

Specificity: The antibody is a rabbit polyclonal antibody raised against APC. It has been selected for its ability to recognize APC in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPC314Mu02Recombinant Adenomatosis Polyposis Coli Protein (APC)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: Adenomatosis Polyposis Coli Protein

Background: DP2, DP2.5, DP3, FAP, FPC, GS, Deleted in polyposis 2.5

UniProt: Q61315

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization