KLRK1 antibody (AA 121-219)

Catalog No. ABIN730078

This Rabbit Polyclonal antibody specifically detects KLRK1 in WB, FACS, ELISA, IHC (fro), IF (cc), IF (p) and IHC (p). It exhibits reactivity toward Mouse and has been mentioned in 3+ publications.

Quick Overview for KLRK1 antibody (AA 121-219) (ABIN730078)

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLRK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KLRK1 Antibody

Binding Specificity: AA 121-219

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat KLRK1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-KLRK1 antibody (ABIN730078)

Mozer-Lisewska, Mania, Kowala-Piaskowska, Kluk, Samara, Pauli, ?eromski: "Detection and significance of cytotoxic cell subsets in biopsies of HCV-infected human livers." in: Archivum immunologiae et therapiae experimentalis, Vol. 62, Issue 2, pp. 153-60, (2014) (PubMed).

Onishi, Kiyota, Koya, Tanaka, Umebayashi, Katano, Morisaki: "Random migration contributes to cytotoxicity of activated CD8+ T-lymphocytes but not NK cells." in: Anticancer research, Vol. 34, Issue 8, pp. 3947-56, (2014) (PubMed).

Ruck, Bittner, Gross, Breuer, Albrecht, Korr, Göbel, Pankratz, Henschel, Schwab, Staszewski, Prinz, Kuhlmann, Meuth, Wiendl: "CD4+NKG2D+ T cells exhibit enhanced migratory and encephalitogenic properties in neuroinflammation." in: PLoS ONE, Vol. 8, Issue 11, pp. e81455, (2013) (PubMed).

Target Details for KLRK1

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: NKG2D

Background:

Synonyms: NKG2D, Nkrp2, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NK lectin-like receptor, NKLLR, NKG2-D-activating NK receptor, NKR-P2, CD314, Klrk1

Background: Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins.

Gene ID: 24934

UniProt: O70215

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process