CH25H antibody

Catalog No. ABIN7636862

The Rabbit Polyclonal anti-CH25H antibody has been validated for WB, IHC, IP, ICC. It is suitable to detect CH25H in samples from Human.

Quick Overview for CH25H antibody (ABIN7636862)

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CH25H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CH25H Antibody

Purpose: Polyclonal Antibody to Cholesterol-25-Hydroxylase (CH25H)

Specificity: The antibody is a rabbit polyclonal antibody raised against CH25H. It has been selected for its ability to recognize CH25H in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CH25H

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Alternative Name: CH25H

Background: H25OH, Cholesterol 25-monooxygenase

UniProt: O95992