GRP94 antibody

Catalog No. ABIN7640063

The Mouse Monoclonal anti-GRP94 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect GRP94 in samples from Human.

Quick Overview for GRP94 antibody (ABIN7640063)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-GRP94 Antibody

Purpose: Monoclonal Antibody to Heat Shock Protein 90kDa Beta 1 (HSP90b1)

Specificity: The antibody is a mouse monoclonal antibody raised against HSP90b1. It has been selected for its ability to recognize HSP90b1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: HSP90b1

Background: HSP90-B1, ECGP, GP96, GRP94, TRA1, Glycoprotein 96, Tumor Rejection Antigen(gp96)1,94 kDa glucose-regulated protein, Endoplasmin

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades