NSF antibody

Catalog No. ABIN7643229

This anti-NSF antibody is a Mouse Monoclonal antibody detecting NSF in WB, IP, IHC and ICC. Suitable for Human.

Quick Overview for NSF antibody (ABIN7643229)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-NSF Antibody

Purpose: Monoclonal Antibody to N-Ethylmaleimide Sensitive Factor (NSF)

Specificity: The antibody is a mouse monoclonal antibody raised against NSF. It has been selected for its ability to recognize NSF in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: N-Ethylmaleimide Sensitive Factor

Background: SKD2, N-Ethylmaleimide Sensitive Fusion Protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF

UniProt: P46459