NEFH antibody

Catalog No. ABIN7643405

The Rabbit Polyclonal anti-NEFH antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect NEFH in samples from Human.

Quick Overview for NEFH antibody (ABIN7643405)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-NEFH Antibody

Purpose: Polyclonal Antibody to Neurofilament, Heavy Polypeptide (NEFH)

Specificity: The antibody is a rabbit polyclonal antibody raised against NEFH. It has been selected for its ability to recognize NEFH in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPE037Hu01Recombinant Neurofilament, Heavy Polypeptide (NEFH)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: NEFH

Background: NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein

UniProt: P12036