NFkB antibody

Catalog No. ABIN7643678

The Mouse Monoclonal anti-NFkB antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect NFkB in samples from Rat.

Quick Overview for NFkB antibody (ABIN7643678)

Target: NFkB (Nuclear Factor kappa B (NFkB))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFkB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NFkB Antibody

Purpose: Monoclonal Antibody to Nuclear Factor Kappa B (NFkB)

Specificity: The antibody is a mouse monoclonal antibody raised against NFkB. It has been selected for its ability to recognize NFkB in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Alternative Name: NFkB

Background: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit

UniProt: Q63369

Pathways: Ubiquitin Proteasome Pathway, S100 Proteins