P4HB antibody

Catalog No. ABIN7644887

The Rabbit Polyclonal anti-P4HB antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect P4HB in samples from Human.

Quick Overview for P4HB antibody (ABIN7644887)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-P4HB Antibody

Purpose: Polyclonal Antibody to Protein Disulfide Isomerase (PDI)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDI. It has been selected for its ability to recognize PDI in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: Protein Disulfide Isomerase

Background: PDI-A2, PDA2, PDIA2, PDIR, PDIP, Protein Disulfide Isomerase,Pancreatic, Protein Disulfide Isomerase-Associated 2, Pancreas-specific protein disulfide isomerase

UniProt: Q13087

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism