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Topoisomerase I antibody

This anti-Topoisomerase I antibody is a Rabbit Polyclonal antibody detecting Topoisomerase I in WB, IHC, IP and ICC. Suitable for Mouse.
Catalog No. ABIN7647234

Quick Overview for Topoisomerase I antibody (ABIN7647234)

Target

See all Topoisomerase I (TOP1) Antibodies
Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity

  • 68
  • 28
  • 16
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Mouse

Host

  • 55
  • 12
  • 2
Rabbit

Clonality

  • 45
  • 24
Polyclonal

Conjugate

  • 42
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
This Topoisomerase I antibody is un-conjugated

Application

  • 45
  • 19
  • 19
  • 17
  • 15
  • 14
  • 7
  • 5
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Purpose

    Polyclonal Antibody to Topoisomerase I (TOP1)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against TOP1. It has been selected for its ability to recognize TOP1 in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

    Alternative Name

    TOP1

    Background

    TOPI, DNA Topoisomerase 1

    UniProt

    Q04750

    Pathways

    Caspase Cascade in Apoptosis, Stem Cell Maintenance
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