YWHAE antibody (CF®488A)

Catalog No. ABIN7649008

This Mouse Monoclonal antibody specifically detects YWHAE in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for YWHAE antibody (CF®488A) (ABIN7649008)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This YWHAE antibody is conjugated to CF®488A

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CPTC-YWHAE-1

Product Details anti-YWHAE Antibody (CF®488A)

Purpose: 14-3-3E / Tryptophan 5-Monooxygenase(CPTC-YWHAE-1), CF488A conjugate

Characteristics: 14-3-3E is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3E has also been identified as a possible susceptibility gene for schizophrenia. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length YWHAE protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HEK-293 or A-431 cell lines.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: 14-3-3E

Background:

Synonyms: YWHAE, Tyrosine 3-Monooxygenase Activation Protein Epsilon, Tryptophan 5-Monooxygenase Activation Protein Epsilon, epididymis luminal protein 2, Mitochondrial import stimulation factor L subunit, protein kinase C inhibitor protein-1

Gene Symbol: YWHAE

Molecular Weight: 29.4 kDa

Gene ID: 7531

UniProt: P62268

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase