Alkaline Phosphatase antibody (CF®740)

Catalog No. ABIN7649374

This Mouse Monoclonal antibody specifically detects Alkaline Phosphatase in IHC and IHC (fp). It exhibits reactivity toward Human and Cow.

Quick Overview for Alkaline Phosphatase antibody (CF®740) (ABIN7649374)

Target: Alkaline Phosphatase (ALP)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Alkaline Phosphatase antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ALPL-597

Product Details anti-Alkaline Phosphatase Antibody (CF®740)

Purpose: Alkaline Phosphatase(ALPL/597), CF740 conjugate

Characteristics: This antibody reacts with tissue non-specific alkaline phosphatase. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ALP1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow Cytometry 5-10 uL/million cells in 0.1 mL. Immunohistology formalin-fixed 1:50-1:100. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunofluorescence 1:50-1:100. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Intestine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase

Background:

Synonyms: Alkaline phosphatase tissue non-specific, Alkaline phosphatase liver/bone/kidney, Alkaline phosphatase biomineralization associated, TNAP, TNALP, ALPL

Gene Symbol: ALPP

Molecular Weight: 55 kDa

Gene ID: 248

UniProt: P10696