ASRGL1 antibody (CF®740)

Catalog No. ABIN7649685

This anti-ASRGL1 antibody is a Mouse Monoclonal antibody detecting ASRGL1 in IHC and IHC (fp). Suitable for Human.

Quick Overview for ASRGL1 antibody (CF®740) (ABIN7649685)

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASRGL1 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CRASH-1289

Product Details anti-ASRGL1 Antibody (CF®740)

Purpose: ASRGL1 (Asparaginase Like 1) (CRASH/1289), CF740 conjugate

Characteristics: ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length ASRGL1

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa cells. Fallopian Tube, Endometrium, Uterus or Cervix.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ASRGL1

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Alternative Name: ASRGL1

Background:

Synonyms: ASRGL1, isoaspartyl peptidase, L-asparaginase, ALP1, beta-aspartyl-peptidase, CRASH, L asparagine amidohydrolase

Gene Symbol: ASRGL1

Molecular Weight: 17-25 kDa

Gene ID: 80150