Recombinant BrdU antibody

Catalog No. ABIN7650161

The Rabbit Monoclonal anti-BrdU antibody has been validated for . It is suitable to detect BrdU in samples from Various Species.

Quick Overview for Recombinant BrdU antibody (ABIN7650161)

Target: BrdU (Bromodeoxyuridine (BrdU))

Antibody Type: Recombinant Antibody

Reactivity: Various Species

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Please inquire

Clone: BRD-1539R

Product Details anti-BrdU Antibody

Purpose: Bromodeoxyuridine (BrdU) (Proliferation Marker)(BRD/1539R)

Characteristics: Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA. This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).

Immunogen: Bromodeoxyuridine (BrdU) conjugated to KLH

Isotype: IgG

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical

Background:

Synonyms: Bromodeoxyuridine, 5-bromo-2-deoxyuridine

Gene Symbol: Not Applicable

Molecular Weight: 307Da