Calcineurin B antibody (CF®488A)

Catalog No. ABIN7650387

This anti-Calcineurin B antibody is a Mouse Monoclonal antibody detecting Calcineurin B in . Suitable for Human and Xenopus laevis.

Quick Overview for Calcineurin B antibody (CF®488A) (ABIN7650387)

Target: Calcineurin B (CAN)

Reactivity: Human, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calcineurin B antibody is conjugated to CF®488A

Application: Please inquire

Clone: BE2-1

Product Details anti-Calcineurin B Antibody (CF®488A)

Purpose: Calcineurin B / PPP3R1(BE2.1), CF488A conjugate

Characteristics: This antibody recognizes the Calcineurin B alpha protein, encoded by the PPP3R1 (Protein Phosphatase 3 Regulatory Subunit B Alpha) gene. Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Calcineurin B protein

Isotype: IgG2a, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HeLa, MCF-7 or A431 cells. Human brain, breast or stomach tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Calcineurin B

Target: Calcineurin B (CAN)

Alternative Name: Calcineurin B

Background:

Synonyms: Calcineurin B, Calcineurin subunit B type 1, Protein Phosphatase 3 Regulatory Subunit B Alpha, CALNB1, Cna2, CNB, CNB1, PPP3R1

Gene Symbol: PPP3R1

Tissue Expression: Brain|T-cells

Molecular Weight: 19 kDa

Gene ID: 5534

UniProt: P63098

Pathways: Cellular Glucan Metabolic Process, VEGF Signaling