CAPN1 antibody (CF®640R)

Catalog No. ABIN7650434

The Mouse Monoclonal anti-CAPN1 antibody has been validated for IHC and IHC (fp). It is suitable to detect CAPN1 in samples from Human.

Quick Overview for CAPN1 antibody (CF®640R) (ABIN7650434)

Target: CAPN1 (Calpain 1 (CAPN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAPN1 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CAPN1-1530

Product Details anti-CAPN1 Antibody (CF®640R)

Purpose: Calpain 1(CAPN1/1530), CF640R conjugate

Characteristics: Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2 - dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CAPN1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa cells. Skin or Tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Alternative Name: Calpain 1

Background:

Synonyms: Calpain 1, Calcium dependent protease small subunit 1, Calcium-activated neutral proteinase 1, Calpain 1 large subunit, Calpain mu-type, Calpain-1 catalytic subunit, CANP1, CANPL1, CAPN1

Gene Symbol: CAPN1

Molecular Weight: ~80 kDa

Gene ID: 823

UniProt: P07384