CTNNA1 antibody (CF®740)

Catalog No. ABIN7650594

This anti-CTNNA1 antibody is a Mouse Monoclonal antibody detecting CTNNA1 in WB. Suitable for Human.

Quick Overview for CTNNA1 antibody (CF®740) (ABIN7650594)

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNA1 antibody is conjugated to CF®740

Application: Western Blotting (WB)

Clone: 1G5

Product Details anti-CTNNA1 Antibody (CF®740)

Purpose: Catenin, alpha-1 (CTNNA1) (1G5), CF740 conjugate

Characteristics: Alpha-E-catenin (also known as Alpha-1 catenin) is part of the catenin family, a large family of Ca2 -dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Alpha-E-catenin is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, alpha-E-catenin forms a heterodimer with beta-catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-E-catenin also regulates the beta-catenin signaling in various cells. It displays the tumor suppressor activity and is found to be down regulated in some forms of breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human alpha catenin fusion protein

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa, A431 or MCF-7 Cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: Alpha-1 catenin

Background:

Synonyms: Alpha-E-catenin, Alpha-1 catenin, Cadherin-associated protein, Catenin alpha-1, CTNNA1

Gene Symbol: CTNNA1

Tissue Expression: Epithelial cells

Molecular Weight: 102 kDa

Gene ID: 1495

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location