CD163 antibody (AA 43-196) (CF®740)

Catalog No. ABIN7650845

The Mouse Monoclonal anti-CD163 antibody has been validated for IHC and IHC (fp). It is suitable to detect CD163 in samples from Human.

Quick Overview for CD163 antibody (AA 43-196) (CF®740) (ABIN7650845)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: M130-2162

Product Details anti-CD163 Antibody (CF®740)

Binding Specificity: AA 43-196

Purpose: CD163 (Monocyte & Macrophage Marker)(M130/2162), CF740 conjugate

Characteristics: This MAb recognizes a protein of 140 kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 43-196) of human CD163

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: hPBL Cells. Skin, Lung, Placenta or Histiocytoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background:

Synonyms: CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130

Gene Symbol: CD163

Tissue Expression: Monocytes/macrophages

Molecular Weight: 140 kDa

Gene ID: 9332