PD-L1 antibody (AA 39-191) (CF®405S)

Catalog No. ABIN7651046

The Mouse Monoclonal anti-PD-L1 antibody has been validated for FACS and IF. It is suitable to detect PD-L1 in samples from Human.

Quick Overview for PD-L1 antibody (AA 39-191) (CF®405S) (ABIN7651046)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PDL1-2743

Product Details anti-PD-L1 Antibody (CF®405S)

Binding Specificity: AA 39-191

Purpose: PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)(PDL1/2743), CF405S conjugate

Characteristics: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human CD274 protein (around aa 39-191)

Isotype: IgG2b, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Flow cytometry: 1-2 μg/mL, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Heart, Placenta, Spleen or Tonsil (IHC). Jurkat cells (IF/FACS).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: B7-H1

Background:

Synonyms: B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1

Gene Symbol: CD274

Molecular Weight: 37-50 kDa

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints