PD-L1 antibody (AA 39-191) (CF®405S)

Catalog No. ABIN7651047

This anti-PD-L1 antibody is a Mouse Monoclonal antibody detecting PD-L1 in WB. Suitable for Human and Mouse.

Quick Overview for PD-L1 antibody (AA 39-191) (CF®405S) (ABIN7651047)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is conjugated to CF®405S

Application: Western Blotting (WB)

Clone: PDL1-2744

Product Details anti-PD-L1 Antibody (CF®405S)

Binding Specificity: AA 39-191

Purpose: PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)(PDL1/2744), CF405S conjugate

Characteristics: Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T-cell proliferation, cytokine production, differentiation of effector T-cells and the induction of BCLX, a promoter of T-cell survival. Engagement of CTLA4 by B7-1 or B7-2, on the other hand, may inhibit proliferation and interleukin-2 (IL-2) production. PD-L1 is 290-amino acid type I transmembrane protein, which is 20 % and 15 % identical to B7-1 and B7-2, respectively, has immunoglobulin V-like and C-like domains and a 30-amino acid cytoplasmic tail. PD-L1 does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of PD-L1 co-stimulation. PD-L2 protein contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the PD-1-PD-L system could provide unique negative signaling to help prevent autoimmune diseases. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human CD274 protein (around aa39-191)

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Jurkat, HEK293 or HepG2 cells. Lung SqCC, Cervical or Breast Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: B7-H1

Background:

Synonyms: B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1

Gene Symbol: CD274

Molecular Weight: 37-50 kDa

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints