CD70 antibody (CF®740)

Catalog No. ABIN7651952

The Mouse Monoclonal anti-CD70 antibody has been validated for FACS and Func. It is suitable to detect CD70 in samples from Human.

Quick Overview for CD70 antibody (CF®740) (ABIN7651952)

Target: CD70 (CD70 Molecule (CD70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD70 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: BU69

Product Details anti-CD70 Antibody (CF®740)

Purpose: CD70(BU69), CF740 conjugate

Characteristics: This antibody recognizes a protein of 30 kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human WM-1 (Waldenstrom s macroglobulinemia) cell line

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (frozen) 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. ELISA For coating, order Ab without BSA. Inhibits T cell proliferation induced by dendritic cells. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Raji cells. Lymph node or tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD70

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD70

Background:

Synonyms: CD27 ligand (CD27L), CD27LG, Ki24, Surface antigen CD70, Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)

Gene Symbol: CD70

Molecular Weight: ~30 kDa

Gene ID: 970

UniProt: P32970

Pathways: Cancer Immune Checkpoints