CD84 antibody (CF®740)

Catalog No. ABIN7652129

The Mouse Monoclonal anti-CD84 antibody has been validated for FACS, WB, IP and Func. It is suitable to detect CD84 in samples from Human.

Quick Overview for CD84 antibody (CF®740) (ABIN7652129)

Target: CD84

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD84 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Clone: 152-1D5

Product Details anti-CD84 Antibody (CF®740)

Purpose: CD84(152-1D5), CF740 conjugate

Characteristics: Recognizes a protein of 74 kDa, identified as CD84 (Workshop V, Code A085, C057). It is expressed on mature B cells and on B-cell lines, including pre-B-cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Spleen cells of a patient with hairy cell leukemia

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Raji, HL60, THP-1, HMC-1, HEL and NAMALWA cells. Human platelets and macrophages. Human tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD84

Target: CD84

Alternative Name: CD84

Background:

Synonyms: CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28

Gene Symbol: CD84

Tissue Expression: B-cells

Molecular Weight: 74 kDa

Gene ID: 8832

UniProt: Q9UIB8