Clathrin, Light Chain (N-Term) antibody (CF®740)

Catalog No. ABIN7652454

This anti-Clathrin, Light Chain antibody is a Mouse Monoclonal antibody detecting Clathrin, Light Chain in IHC and IHC (fp). Suitable for Human.

Quick Overview for Clathrin, Light Chain (N-Term) antibody (CF®740) (ABIN7652454)

Target: Clathrin, Light Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CLC-1421

Product Details

Binding Specificity: N-Term

Purpose: Clathrin, Light Chain (CLC/1421), CF740 conjugate

Characteristics: This antibody recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant N-terminal fragment of human Clathrin Light Chain

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa cells. Placenta or Prostate Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Clathrin, Light Chain

Background:

Synonyms: Clathrin light chain A, Clathrin light chain B, Clathrin light chain LCA, Clathrin light chain LCB, Clathrin light polypeptide A, Clathrin light polypeptide B, Clathrin, light polypeptide (Lca), Clathrin, light polypeptide (Lcb), CLTA, CLTB

Gene Symbol: CLTA|CLTB

Molecular Weight: 31-44 kDa

Gene ID: 1211, 1212

UniProt: P09496, P09497