FAT Atypical Cadherin 2 (FAT2) antibody (CF®740)

Catalog No. ABIN7653924

This anti- antibody is a Mouse Monoclonal antibody detecting in IHC and IHC (fp). Suitable for Human.

Quick Overview for FAT Atypical Cadherin 2 (FAT2) antibody (CF®740) (ABIN7653924)

Target: FAT Atypical Cadherin 2 (FAT2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 8C5

Product Details

Purpose: Protocadherin FAT2 (FAT Atypical Cadherin 2) (8C5), CF740 conjugate

Characteristics: This antibody recognizes a protein of 480 kDa, which is identified as FAT2. The cadherins represent a family of Ca2 -dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant human FAT2 fusion protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Esophagus, Cervix, Uterus, Tonsil or Cerebellum.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: FAT Atypical Cadherin 2 (FAT2)

Alternative Name: Protocadherin-FAT2

Background:

Synonyms: FAT tumor suppressor homolog 2 (HFAT2), cadherin family member 8 (CDHF8), cadherin-related family member 9 (CDHR9), multiple EGF-like domains protein 1, multiple epidermal growth factor-like domains 1 (MEGF1), multiple epidermal growth factor-like domains protein 1, protocadherin FAT2

Gene Symbol: FAT2

Molecular Weight: 480 kDa

Gene ID: 2196

UniProt: Q9NYQ8