GAD antibody (AA 72-135) (CF®640R)

Catalog No. ABIN7654421

The Mouse Monoclonal anti-GAD antibody has been validated for FACS. It is suitable to detect GAD in samples from Human.

Quick Overview for GAD antibody (AA 72-135) (CF®640R) (ABIN7654421)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD antibody is conjugated to CF®640R

Application: Flow Cytometry (FACS)

Clone: GAD1-2563

Product Details anti-GAD Antibody (CF®640R)

Binding Specificity: AA 72-135

Purpose: GAD1 / GAD67 (GABAergic Neuronal Marker) (GAD1/2563), CF640R conjugate

Characteristics: This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (AŽ³-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135)

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: For coating order antibody without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Western: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: K-562 or HEK293 Cells. Pancreas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1

Background:

Synonyms: 67 kDa glutamic acid decarboxylase, CPSQ1, DCE1, GAD67, GAD1, Glutamate decarboxylase 1, SCP

Gene Symbol: GAD1

Tissue Expression: Brain

Molecular Weight: 67 kDa

Gene ID: 2571

UniProt: Q99259