Recombinant ATRX antibody (CF®568)

Catalog No. ABIN7654869

This anti-ATRX antibody is a Rabbit Monoclonal antibody detecting ATRX in IHC and IHC (fp). Suitable for Human.

Quick Overview for Recombinant ATRX antibody (CF®568) (ABIN7654869)

Target: ATRX (helicase 2, X-linked (ATRX))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ATRX antibody is conjugated to CF®568

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ATRX-2900R

Product Details anti-ATRX Antibody (CF®568)

Purpose: ATRX / RAD54 (Alpha Thalassemia Mental Retardation) (ATRX/2900R), CF568 conjugate

Characteristics: ATRX is a member of the Snf2 family of helicase/ATPases, which contribute to the remodeling of the nucelosome structure in an ATP-dependent manner, and facilitate the initiation of transcription and replication. Structurally, ATRX contains a PHD zinc finger motif. ATRX is regulated throughout the cell cycle where it is differentially distributed within the nucleus. During interphase, ATRX predominately associates with the nuclear matrix, while during mitosis, ATRX localizes with condensed chromatin. At the onset of M phase, phosphorylation rapidly induces this redistribution of ATRX to the short arms of human acrocentric chromosomes, where it then specifically complexes with heterochromatin protein 1 alpha to mediate chromosomal segregation. Mutations in the ATRX gene correlate with a high incidence of severe X-linked form of syndromal mental retardation associated with alpha-thalassemia or ATRX syndrome. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human ATRX protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Raji, HeLa or A431 Cells. Pancreas, Adrenal, Ovary, Kidney, Prostate or Stomach.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ATRX

Target: ATRX (helicase 2, X-linked (ATRX))

Alternative Name: ATRX

Background:

Synonyms: Transcriptional regulator ATRX, ATP-dependent helicase ATRX, RAD54 Homolog, X-linked helicase II, X-linked nuclear protein

Gene Symbol: ATRX

Molecular Weight: 280 kDa

Gene ID: 546

UniProt: P46100