TIM3 antibody (AA 22-202)

Catalog No. ABIN7654951

This Mouse Monoclonal antibody specifically detects TIM3 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for TIM3 antibody (AA 22-202) (ABIN7654951)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIM3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: HAVCR2-192

Product Details anti-TIM3 Antibody

Binding Specificity: AA 22-202

Purpose: TIM3 / HAVCR2 / CD366 (Effector T-Cell Marker)(HAVCR2/192)

Characteristics: TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cells (CD4 Th1 and CD8 Tc1) but not on helper T cells (CD4 Th2 and CD8 Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human TIM3 protein (around aa 22-202)

Isotype: IgG2a, kappa

Application Details

Application Notes: ELISA: For coating, order Ab without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: PC3, BT474, HepG2, HDLM-2 or Daudi cells. Tonsil, lymph node or spleen.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: CD366

Background:

Synonyms: CD366, HAVR2, Hepatitis A virus cellular receptor 2 (HAVCR2), Kidney injury molecule 3 (KIM3), T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM3, TIMD3

Gene Symbol: HAVCR2

Molecular Weight: 60 kDa

Gene ID: 84868

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints