KRT17 antibody (CF®740)

Catalog No. ABIN7656020

The Mouse Monoclonal anti-KRT17 antibody has been validated for WB, IHC, IF, FACS and IHC (fp). It is suitable to detect KRT17 in samples from Human, Rat, Cow, Pig and Goat.

Quick Overview for KRT17 antibody (CF®740) (ABIN7656020)

Target: KRT17 (Keratin 17 (KRT17))

Reactivity: Human, Rat, Cow, Pig, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT17 antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: E3

Product Details anti-KRT17 Antibody (CF®740)

Purpose: Cytokeratin 17(E3), CF740 conjugate

Characteristics: Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered asepithelial stem cellmarker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: The cytoskeletal fraction of rat colon epithelium

Isotype: IgG2b, kappa

Application Details

Application Notes: Immunohistology frozen & formalin-fixed 0.5-1 ug/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 ug/million cells/0.1 mL. Immunofluorescence 1-2 ug/mL. Western blotting 0.5-1 ug/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: T24 cells or Skin. Bladder or Cervix.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for KRT17

Target: KRT17 (Keratin 17 (KRT17))

Alternative Name: Keratin 17

Background:

Synonyms: K17, Keratin Type I Cytoskeletal 17, Keratin-17, KRT17, PCHC1

Gene Symbol: KRT17

Tissue Expression: Epithelial cells

Molecular Weight: 46 kDa

Gene ID: 3872

UniProt: Q04695