Ku70/80 (Holo) antibody (CF®740)

Catalog No. ABIN7656310

This Mouse Monoclonal antibody specifically detects in IF and FACS. It exhibits reactivity toward Human and Non-Human Primate.

Quick Overview for Ku70/80 (Holo) antibody (CF®740) (ABIN7656310)

Target: Ku70/80

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®740

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: KU729

Product Details

Binding Specificity: Holo

Purpose: Ku-Holo (p70,83)(KU729), CF740 conjugate

Characteristics: Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Nuclear extract of human HL-60 cells

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Does not react with mouse, rat or chicken, others not known. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Ku70/80

Background:

Synonyms: Ku (p70): 70 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, Ku autoantigen, 70 kDa, Ku70, Kup70, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80): 86 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 2, ATP dependent DNA helicase II 86Kd subunit, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, KARP1, Ku autoantigen 80 kDa, Ku80, Ku86 autoantigen related protein 1, KUB2, Lupus Ku autoantigen protein p86, Nuclear factor IV (NFIV), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 5 (XRCC5)

Gene Symbol: XRCC6

Molecular Weight: 70 kDa & 80 kDa

Gene ID: 2547