L1CAM antibody (CF®740)

Catalog No. ABIN7656311

The Mouse Monoclonal anti-L1CAM antibody has been validated for WB, IHC, FACS, IP and IHC (fp). It is suitable to detect L1CAM in samples from Human.

Quick Overview for L1CAM antibody (CF®740) (ABIN7656311)

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This L1CAM antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: UJ127

Product Details anti-L1CAM Antibody (CF®740)

Purpose: CD171 / L1CAM (L1 Cell Adhesion Molecule)(UJ127), CF740 conjugate

Characteristics: Recognizes a cell surface protein of 220-240 kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius), 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs), and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This MAb is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Homogenous suspension of 16 week human fetal brain

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Neuroblastomas or Schwannomas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Alternative Name: CD171

Background:

Synonyms: CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1

Gene Symbol: L1CAM

Molecular Weight: 220-240 kDa

Gene ID: 3897

UniProt: P32004

Pathways: Synaptic Membrane