LGALS13 antibody (AA 23-134) (CF®740)

Catalog No. ABIN7656326

The Mouse Monoclonal anti-LGALS13 antibody has been validated for IHC and IHC (fp). It is suitable to detect LGALS13 in samples from Human.

Quick Overview for LGALS13 antibody (AA 23-134) (CF®740) (ABIN7656326)

Target: LGALS13 (Lectin, Galactoside-Binding, Soluble, 13 (LGALS13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGALS13 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PP13-1162

Product Details anti-LGALS13 Antibody (CF®740)

Binding Specificity: AA 23-134

Purpose: Galectin-13 (GAL13) / Placental Protein 13 (PP13) (PP13/1162), CF740 conjugate

Characteristics: This antibody recognizes a 32 kDa protein, which is identified as homodimer of galectin-13 (also known as PP13). Galectins are a family of soluble beta-galactoside-binding lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One such member, galectin-13, is a 139 amino acid protein that contains one galectin domain. Possessing lysophospholipase activity, galectin-13 exists as a disulfide-linked homodimer. Galectin-13 is suggested to have a developmental role in the placenta and may display immunobiological activity in feto-maternal blood-spaces. Screening for galectin-13 in maternal serum during the first trimester of pregnancy may serve as a diagnostic marker to predict preterm pre-eclampsia. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Galectin-13 protein fragment (aa23-134)

Isotype: IgG2b

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 or 10 mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: K-562 cells. Placenta or Spleen.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for LGALS13

Target: LGALS13 (Lectin, Galactoside-Binding, Soluble, 13 (LGALS13))

Alternative Name: Galectin-13

Background:

Synonyms: Gal-13, GAL13, Galactoside-binding soluble lectin 13, Galectin-13, Galectin13, Lectin galactoside binding soluble 13, LGALS13, PLAC8, Placental protein 13 (PP13), Placental tissue protein 13

Gene Symbol: LGALS13

Molecular Weight: 16 kDa (monomer), 32 kDa (homodimer)

Gene ID: 29124

UniProt: Q9UHV8