MUC5AC antibody (CF®740)

Catalog No. ABIN7657435

This Mouse Monoclonal antibody specifically detects MUC5AC in WB, IHC, FACS, ELISA and IHC (fp). It exhibits reactivity toward Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken, Pig and Hedgehog.

Quick Overview for MUC5AC antibody (CF®740) (ABIN7657435)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity: Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken, Pig, Hedgehog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5AC antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 45M1

Product Details anti-MUC5AC Antibody (CF®740)

Purpose: Mucin 5AC (MUC5AC)(45M1), CF740 conjugate

Characteristics: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. The epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 1-2 μg/mL. Does not react with cow, others not known. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Stomach

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Alternative Name: Mucin 5AC

Background:

Synonyms: Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Gene Symbol: MUC5AC

Tissue Expression: Epithelial cells

Molecular Weight: >1,000 kDa

Gene ID: 4586

UniProt: P98088