MUC5AC antibody (CF®488A)

Catalog No. ABIN7657448

The Mouse Monoclonal anti-MUC5AC antibody has been validated for WB and RIA. It is suitable to detect MUC5AC in samples from Human, Mouse, Monkey, Cat and Cow.

Quick Overview for MUC5AC antibody (CF®488A) (ABIN7657448)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity: Human, Mouse, Monkey, Cat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5AC antibody is conjugated to CF®488A

Application: Western Blotting (WB), Radioimmunoassay (RIA)

Clone: 2-11M1

Product Details anti-MUC5AC Antibody (CF®488A)

Purpose: MUC5AC (Mucin 5AC / Gastric Mucin)(2-11M1), CF488A conjugate

Characteristics: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb 2-11M1 reacts with the protein backbone exclusively, it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then 2-11M1 will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: Use at 2-4 μg/mL for coating. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Western blot: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: MCF-7 cells. Human colon or stomach (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Alternative Name: MUC5AC

Background:

Synonyms: Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Gene Symbol: MUC5AC

Tissue Expression: Epithelial cells

Molecular Weight: >1,000 kDa

Gene ID: 4586

UniProt: P98088