Recombinant pan Keratin antibody (CF®647)

Catalog No. ABIN7658406

This anti-pan Keratin antibody is a Mouse Monoclonal antibody detecting pan Keratin in IHC and IHC (fp). Suitable for Human, Rat, Mouse, Cow, Monkey, Pig, Frog, Guinea Pig and Goat.

Quick Overview for Recombinant pan Keratin antibody (CF®647) (ABIN7658406)

Target: pan Keratin (panKRT)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse, Cow, Monkey, Pig, Frog, Guinea Pig, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RKRT-457

Product Details anti-pan Keratin Antibody (CF®647)

Purpose: Cytokeratin, Multi (Epithelial Marker)(rKRT/457), CF647 conjugate

Characteristics: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6. 0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56. 5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Keratin-enriched preparation from cultured human epithelial cells

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: A431 cells, Skin, Colon carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Keratin, Multi

Background:

Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Tissue Expression: Epidermal cells|Epithelial cells

Molecular Weight: Multiple

Gene ID: 3851, 3852, 3853

UniProt: P02538, P04259, P13647, P19013, P48668