PTH antibody (C-Term) (CF®740)

Catalog No. ABIN7658491

This Mouse Monoclonal antibody specifically detects PTH in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for PTH antibody (C-Term) (CF®740) (ABIN7658491)

Target: PTH (Parathyroid Hormone (PTH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTH antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 3H9 PTH-1175

Product Details anti-PTH Antibody (CF®740)

Binding Specificity: C-Term

Purpose: PTH (Parathyroid Hormone) (N & C Terminal) (3H9 + PTH/1175), CF740 conjugate

Characteristics: The epitope of this MAb maps in the C-terminus of PTH, a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood calcium levels by dissolving the salts in bone and preventing their renal excretion.It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Synthetic peptide corresponding to amino acids 1 to 34 of PTH conjugated to a carrier (3H9), Recombinant fragment (84 amino acid residues from C-terminus) of human PTH protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Human parathyroid gland carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PTH

Target: PTH (Parathyroid Hormone (PTH))

Alternative Name: PTH

Target Type: Hormone

Background:

Synonyms: hPTH, Parathormone, Parathyrin, Parathyroid hormone 1 (PTH1), Parathyroid hormone (PTH)

Gene Symbol: PTH

Molecular Weight: 9 kDa

Gene ID: 5741

UniProt: P01270

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process