ADRP antibody (AA 249-376) (CF®740)

Catalog No. ABIN7658561

The Mouse Monoclonal anti-ADRP antibody has been validated for IHC and IHC (fp). It is suitable to detect ADRP in samples from Human.

Quick Overview for ADRP antibody (AA 249-376) (CF®740) (ABIN7658561)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADRP antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ADFP-1493

Product Details anti-ADRP Antibody (CF®740)

Binding Specificity: AA 249-376

Purpose: Adipophilin / Perilipin-2 (Marker of Obesity) (ADFP/1493), CF740 conjugate

Characteristics: Perilipin-2, also known as Adipophilin, belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This 48 kDa protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Adipophilin protein fragment (aa249-376)

Isotype: IgG2b, lambda

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Alternative Name: Adipophilin

Background:

Synonyms: Perilipin-2, Adipophilin, ADFP, ADRP, Adipose differentiation-related protein, Perilipin-2

Gene Symbol: PLIN2

Molecular Weight: 48 kDa

Gene ID: 123

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism