PLAP antibody (CF®740)

Catalog No. ABIN7658622

This anti-PLAP antibody is a Mouse Monoclonal antibody detecting PLAP in WB, IHC and IHC (fp). Suitable for Human.

Quick Overview for PLAP antibody (CF®740) (ABIN7658622)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAP antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PL8-F6

Product Details anti-PLAP Antibody (CF®740)

Purpose: PLAP (Placental Alkaline Phosphatase)(PL8-F6), CF740 conjugate

Characteristics: Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human PLAP protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Immunohistology Frozen & formalin-fixed 0.25-0.5 ug/mL. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues. Immunofluorescence 0.5-1 ug/mL. Flow Cytometry 0.5-1 ug/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HepG2 cells. Placenta or seminoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: PLAP

Background:

Synonyms: Alkaline phosphatase placental type|Alkaline phosphatase Regan Isozyme|ALP|Alp1|ALPP|Germ-cell alkaline phosphatase|nagao Isozyme|PALP|Placental alkaline phosphatase 1|placental heat-stable alkaline phosphatase|PLAP-1|PLAP1

Gene Symbol: ALPP

Tissue Expression: Placenta

Molecular Weight: 70 kDa

Gene ID: 250

UniProt: P05187