Plasma Cell Marker antibody (CF®740)

Catalog No. ABIN7658624

The Mouse Monoclonal anti- antibody has been validated for IHC, IHC (fp) and IF. It is suitable to detect in samples from Human.

Quick Overview for Plasma Cell Marker antibody (CF®740) (ABIN7658624)

Target: Plasma Cell Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunofluorescence (IF)

Clone: LIV3G11

Product Details

Purpose: Plasma Cell Marker(LIV3G11, same as 7B18), CF740 conjugate

Characteristics: This antibody recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4 lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm, their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This MAb superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this MAb is not suitable for staining frozen tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Pancreatic cancer-related mucin

Isotype: IgG2a

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with rat, others not known. Immunohistology formalin-fixed 0.1-0.2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Plasma Cell Marker

Background:

Synonyms: Plasma B cell, Plasmocytes

Tissue Expression: B-cells|Lymphocytes

Molecular Weight: Not Known