PIP antibody (AA 41-146) (CF®740)

Catalog No. ABIN7659191

The Mouse Monoclonal anti-PIP antibody has been validated for WB, IHC and IHC (fp). It is suitable to detect PIP in samples from Human.

Quick Overview for PIP antibody (AA 41-146) (CF®740) (ABIN7659191)

Target: PIP (Prolactin-Induced Protein (PIP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIP antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PIP-1571

Product Details anti-PIP Antibody (CF®740)

Binding Specificity: AA 41-146

Purpose: GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker) (PIP/1571), CF740 conjugate

Characteristics: This antibody recognizes a protein of 15 kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50 % of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GCDFP-15 protein fragment (aa 41-146)

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HepG2 Cells. Breast or Pancreas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PIP

Target: PIP (Prolactin-Induced Protein (PIP))

Alternative Name: GCDFP-15

Background:

Synonyms: BRST-2, GCDFP-15, gp17, GPIP4, Gross cystic disease fluid protein 15, Prolactin-induced protein (PIP), Prolactin-inducible protein (PIP), Secretory actin-binding protein (SABP)

Gene Symbol: PIP

Molecular Weight: 15 kDa

Gene ID: 5304

UniProt: P12273