ROR gamma antibody (CF®594)

Catalog No. ABIN7659669

This anti- antibody is a Mouse Monoclonal antibody detecting in . Suitable for Human.

Quick Overview for ROR gamma antibody (CF®594) (ABIN7659669)

Target: ROR gamma

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®594

Application: Please inquire

Clone: RORC-2942

Product Details

Purpose: ROR-gamma / RORC (RAR-related Orphan Receptor C)(RORC/2942), CF594 conjugate

Characteristics: This MAb recognizes a protein of 63 kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors ROR and ROR are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50 % amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered orphan receptors, due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human RORC protein

Isotype: IgG2a, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Human Lymphocytes. Liver or skeletal muscle tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: ROR gamma

Alternative Name: ROR-Gamma

Background:

Synonyms: Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3 (NR1F3), RAR related orphan receptor C, Retinoid-related orphan receptor-gamma (RORG), Rorc, RZR GAMMA, RZRG, TOR

Gene Symbol: RORC

Molecular Weight: 63 kDa

Gene ID: 6097

UniProt: P51449