Recombinant s100a4 antibody (AA 1-200) (CF®640R)

Catalog No. ABIN7659698

The Rabbit Monoclonal anti-s100a4 antibody has been validated for WB, IHC, IF, FACS and IHC (fp). It is suitable to detect s100a4 in samples from Human and Mouse.

Quick Overview for Recombinant s100a4 antibody (AA 1-200) (CF®640R) (ABIN7659698)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This s100a4 antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: S100A4-2750R

Product Details anti-s100a4 Antibody (CF®640R)

Binding Specificity: AA 1-200

Purpose: S100A4 (Marker of Tumor Metastasis) (S100A4/2750R), CF640R conjugate

Characteristics: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, alpha6beta4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human S100A4 protein (around aa1-200)

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa, A549 or A375 Cells. Placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4

Background:

Synonyms: S100A4, S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein

Gene Symbol: S100A4

Molecular Weight: 10-12 kDa

Gene ID: 6275

UniProt: P26447

Pathways: S100 Proteins