TIMP1 antibody (AA 72-82)

Catalog No. ABIN7660857

This anti-TIMP1 antibody is a Mouse Monoclonal antibody detecting TIMP1 in WB, IHC and IHC (fp). Suitable for Human.

Quick Overview for TIMP1 antibody (AA 72-82) (ABIN7660857)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 2A5

Product Details anti-TIMP1 Antibody

Binding Specificity: AA 72-82

Purpose: TIMP1 (Marker of Lymph Node Metastasis)(2A5)

Characteristics: TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases -1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 is identical to EPA (erythroid-potentiation activity). PTH has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart tissues. Studies have demonstrated that TIMP1 is useful as a biomarker for early detection of colorectal cancer, outperforming CEA. Additionally, TIMP1 studies have demonstrated its role in CRC tumorigenesis, as well as observing its overexpression in metastatic lymph nodes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide (aa 72-82) of human TIMP1 protein (FQALGDAADIR)

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: Use Ab at 2-4 μg/mL for coating, Order Ab without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: 293 cells. Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP1

Background:

Synonyms: Collagenase inhibitor, EPO, Erythroid-Potentiating Activity (EPA), Fibroblast collagenase inhibitor, Human Collagenase Inhibitor (HCI), Metalloproteinase inhibitor 1, Tissue Inhibitor of Metalloproteinase 1 (TIMP1)

Gene Symbol: TIMP1

Molecular Weight: 23 kDa

Gene ID: 7076

UniProt: P01033