Transferrin antibody

Catalog No. ABIN7661012

The Mouse Monoclonal anti-Transferrin antibody has been validated for IHC and IHC (fp). It is suitable to detect Transferrin in samples from Human, Rat and Mouse.

Quick Overview for Transferrin antibody (ABIN7661012)

Target: Transferrin (TF)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: A78-G-A7

Product Details anti-Transferrin Antibody

Purpose: Thomsen-Friedenreich Antigen / CD176 (Pan Carcinoma Marker)(A78-G/A7)

Characteristics: This antibody recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide. This antibody is available purified, with BSA and azide (0.2 mg/mL) or purified, BSA-free and azide-free (1 mg/mL).

Immunogen: Neuraminidase-treated human red blood cells

Isotype: IgM, kappa

Application Details

Application Notes: Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: KG1 cells. Human colorectal carcinoma tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: CD176

Background:

Synonyms: T-F Antigen, TF Antigen, Asialoglycophorin, pan-carcinoma marker, CD176

Gene Symbol: Not Applicable

Molecular Weight: Multiple

Pathways: Transition Metal Ion Homeostasis