C-Ski/V-Ski (AA 551-650) antibody

Catalog No. ABIN732938

The Rabbit Polyclonal anti- antibody has been validated for WB, IF (cc), IF (p), ELISA, IHC (fro) and IHC (p). It is suitable to detect in samples from Human.

Quick Overview for C-Ski/V-Ski (AA 551-650) antibody (ABIN732938)

Target: C-Ski/V-Ski

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 551-650

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human C-SKI

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: C-Ski/V-Ski

Background:

Synonyms: Sloan-kettering viral oncogene homolog, Avian sarcoma viral v ski oncogene homolog, C oncogene, C ski, Ski oncogene, Ski oncoprotein, SKV, v ski avian sarcoma viral oncogene homolog, v ski sarcoma viral oncogene homolog, v-ski sarcoma viral oncogene homolog, SKI_HUMAN.

Background: SKI is a nuclear protein that forms homodimers and heterodimers and binds to DNA to function as transcriptional activators and repressors. The SKI oncoprotein dramatically affects cell growth, differentiation, and/or survival. SKI was shown to act in distinct signaling pathways including those involving nuclear receptors, transforming growth factor beta, and tumor suppressors. SKI inhibits transforming growth factor-beta (TGF-beta) signaling through interaction with Smad proteins. SKI represses Smad-mediated transcriptional activation, probably through its action as a transcriptional co-repressor. SKI also inhibits TGF-beta-induced downregulation of genes such as c-myc.

Gene ID: 6497